Publications
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2011
Sonja Wilke ,
Lothar Groebe ,
Vitali Maffenbeier ,
Volker Jäger ,
Manfred Gossen ,
Jörn Josewski ,
Agathe Duda ,
Lilia Polle ,
Raymond J. Owens ,
Dagmar Wirth,
Dirk W. Heinz,
Joop van den Heuvel,
Konrad Büssow
Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development
PLoS One 2011, 6(12):e27829
Jens C de Groot,
Kai Schlüter,
Yvonne Carius,
Claudia Quedenau,
Didier Vingadassalom,
Jan Faix,
Stefanie M Weiss,
Joachim Reichelt,
Christine Standfuß-Gabisch,
Cammie F Lesser,
John M Leong,
Dirk W Heinz,
Konrad Büssow*,
Theresia EB Stradal*
Structural Basis for Complex Formation between Human IRSp53 and the Translocated Intimin Receptor Tir of Enterohemorrhagic E. coli
Structure 2011, 19(9):1294-1306
D. Busso, Y. Peleg, T. Heidebrecht, C. Romier, Y. Jacobovitch, A. Dantes, L. Salim, E. Troesch, A. Schuetz, U. Heinemann, G.E. Folkers, A. Geerlof, M. Wilmanns, A. Polewacz, C. Quedenau, K. Bussow, R. Adamson, E. Blagova, J. Walton, J.L. Cartwright, L.E. Bird, R.J. Owens, N.S. Berrow, K.S. Wilson, J.L. Sussman, A. Perrakis and P.H. Celie
Expression of protein complexes using multiple Escherichia coli protein co-expression systems: A benchmarking study
Journal of Structural Biology 2011, 175(2):159-170
2010
Sonja Wilke, Joern Krausze, Manfred Gossen, Lothar Groebe, Volker Jäger,
Ermanno Gherardi, Joop van den Heuvel and Konrad Büssow
Glycoprotein production for structure analysis with stable, glycosylation mutant CHO cell lines established by fluorescence-activated cell sorting
Protein Science 2010, 19:1264-1271
2008
Jörg O. Schulze, Claudia Quedenau, Yvette Roske, Thomas Adam, Herwig Schüler,
Joachim Behlke, Andrew P. Turnbull, Volker Sievert, Christoph Scheich, Uwe Mueller,
Udo Heinemann and Konrad Büssow
Structural and functional characterization of human Iba proteins
FEBS Journal 2008, 275:4627-4640
Volker Sievert, Asgar Ergin and Konrad Büssow
High throughput cloning with restriction enzymes
Methods in Molecular Biology 2008, 426:163-173.
Babu A. Manjasetty, Andrew P. Turnbull, Santosh Panjikar, Konrad Büssow
and Mark R. Chance
Automated technologies and novel techniques to accelerate protein crystallography for structural genomics
Proteomics 2008, 8(4): 612-625.
2007
Sabine Cepok, Bernhard Hemmer, Konrad Büssow
Identification of Target Antigens in CNS Inflammation by Protein Array Technique
in Bioarrays - From Basics to Diagnostics. Krishnarao Appasani (Ed.), 2007.
Asgar Ergin, Konrad Büssow, Joachim Sieper, Andreas Thiel, Rainer Duchmann and Thomas Adam
Homologous high-throughput expression and purification of highly conserved E coli proteins
Microbial Cell Factories 2007, 6:18.
Christoph Scheich, Daniel Kümmel, Dimitri Soumailakakis,
Udo Heinemann and Konrad Büssow
Vectors for co-expression of an unrestricted number of proteins
Nucleic Acids Research 2007, doi: 10.1093/nar/gkm067
2006
N. S. Berrow, K. Büssow, B. Coutard, J. Diprose, M. Ekberg, G. E. Folkers, N. Levy, V. Lieu, R. J. Owens,
Y. Peleg, C. Pinaglia, S. Quevillon-Cheruel, L. Salim, C. Scheich, R. Vincentelli and D. Busso
Recombinant protein expression and solubility screening in Escherichia coli: a comparative study
Acta Crystallographica Section D 2006, 62 (10): 1218-1226
Babu A. Manjasetty, Konrad Büssow, Martin Fieber-Erdmann, Yvette Roske, Johan Gobom, Christoph Scheich,
Frank Götz, Frank H. Niesen and Udo Heinemann
Crystal structure of Homo sapiens PTD012 reveals a zinc-containing hydrolase fold
Protein Science 2006, 15:914-920
Claus Hultschig, Jürgen Kreutzberger, Harald Seitz, Zoltán Konthur, Konrad Büssow and Hans Lehrach
Recent advances of protein microarrays
Current Opinion in Chemical Biology 2006, 10(1):4-10.
2005
Babu A Manjasetty, Frank H Niesen, Christoph Scheich, Yvette Roske, Frank Götz,
Joachim Behlke, Volker Sievert, Udo Heinemann and Konrad Büssow
X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1)
BMC Structural Biology 2005, 5:21.
Konrad Büssow, Christoph Scheich, Volker Sievert, Ullrich Harttig,
Jörg Schultz, Bernd Simon, Peer Bork, Hans Lehrach, Udo Heinemann
Structural Genomics of human proteins - target selection and generation of a
public catalog of expression clones
Microbial Cell Factories 2005, 4:21.
Daniel Kümmel, Jürgen J. Müller, Yvette Roske, Rolf Misselwitz, Konrad Büssow, Udo Heinemann
The structure of the TRAPP subunit TPC6 suggests a model for a TRAPP subcomplex
EMBO reports 2005, 6(8):787-793
Sabine Cepok, Dun Zhou, Rajneesh Srivastava, Stefan Nessler, Susanne Stei,
Konrad Büssow, Norbert Sommer, and Bernhard Hemmer
Identification of Epstein-Barr virus proteins as putative targets
of the immune response in multiple sclerosis
Journal of Clinical Investigations 2005, 115(5):1352-60.
Hendrik Weiner, Jörg Glökler, Claus Hultschig, Konrad Büssow and Gerald Walter
Protein, antibody and small molecule microarrays,
in MICROARRAY TECHNOLOGY AND ITS APPLICATIONS. ed Muller UR, Nicolau DV, Springer-Verlag Berlin, PG. 279-295. 2005.
2004
Michael Soukenik, Anne Diehl, Martina Leidert, Volker Sievert, Konrad Büssow,
Dietmar Leitner, Dirk Labudde, Linda J. Ball, Annette Lechner, Dorit K. Nägler, Hartmut Oschkinat
The SEP domain of p47 acts as a reversible competitive inhibitor of cathepsin L.
FEBS Letters 2004, 576(3):358-362.
Babu A Manjasetty, Frank H Niesen, Heinrich Delbrück, Frank Götz,
Volker Sievert, Konrad Büssow, Joachim Behlke and Udo Heinemann
X-ray structure of Fumarylacetoacetate Hydrolase(FAH) family member Homo sapiens
FLJ36880.
Biological Chemistry 2004, 385(10):935-942.
H. Goehler, M. Lalowski, U. Stelzl, S. Waelter, M. Stroedicke, U. Worm, A. Droege,
K. S. Lindenberg, M. Knoblich, C. Haenig, M. Herbst, J. Suopanki, E. Scherzinger,
C. Abraham, B. Bauer, R. Hasenbank, A. Fritzsche, A. H. Ludewig, K. Buessow,
S. H. Coleman, C. A. Gutekunst, B. G. Landwehrmeyer, H. Lehrach and E. E. Wanker
A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease
Molecular Cell 2004, 15(6):853-865.
Konrad Büssow, Claudia Quedenau, Volker Sievert, Janett Tischer, Christoph Scheich,
Harald Seitz, Brigitte Hieke, Frank H. Niesen, Frank Götz, Ulrich Harttig and Hans Lehrach
A catalogue of human cDNA expression clones and its application to structural genomics.
Genome Biology 2004, 5:R71.
Hendrik Weiner, Thomas Faupel and Konrad Büssow
Protein arrays for cDNA expression libraries.
Methods in Molecular Biology 2004, 278:1-14.
Christoph Scheich, Dietmar Leitner, Volker Sievert, Martina Leidert,
Brigitte Schlegel, Bernd Simon, Ivica Letunic, Konrad Büssow
and Anne Diehl
Fast identification of folded human protein domains
expressed in E. coli suitable for structural analysis
BMC Structural Biology 2004, 4:4
Christoph Scheich, Frank Niesen, Robert Seckler and Konrad Büssow
An automated in vitro protein folding screen applied to a human dynactin
subunit
Protein Science 2004, 13: 370-380
Babu A. Manjasetty, Claudia Quedenau, Volker Sievert, Konrad Büssow, Frank Niesen, Heinrich Delbrück, Udo Heinemann
X-ray structure of human gankyrin, the product of a gene linked to hepatocellular carcinoma
Proteins: Structure, Function, and Bioinformatics 2004, 55(1):214-217
Christoph Brockmann,
Dietmar Leitner,
Dirk Labudde,
Anne Diehl,
Volker Sievert,
Konrad Büssow,
Ronald Kühne and
Hartmut Oschkinat
The solution structure of the SODD BAG domain reveals additional electrostatic interactions in the HSP70 complexes of SODD subfamily BAG domains
FEBS Letters 2004 558(1-3):101-106
Katrin de Graaf, Paul Hekerman, Oliver Spelten, Andreas Herrmann, Len C. Packman, Konrad Büssow, Gerhard Müller-Newen, and Walter Becker
Characterization of cyclin L2, a novel cyclin with an arginine/serine-rich (RS) domain: Phosphorylation by DYRK1A and colocalization with splicing factors.
Journal of Biological Chemistry 2004, 279:4612-4624
Uwe Mueller, Konrad Büssow, Anne Diehl, Franz J. Bartl, Frank H. Niesen, Lajos Nyarsik and Udo Heinemann
Rapid Purification and crystal structure analysis of a small protein
carrying two terminal affinity tags.
Journal of Structural and Functional Genomics 2004, 4: 217-225
Babu A Manjasetty, Heinrich Delbrück, Dinh Trung Pham, Uwe Mueller, Martin
Fieber-Erdmann, Christoph Scheich, Volker Sievert, Konrad Büssow,
Frank Niesen, Wilhelm Weihofen, Bernhard Loll, Wolfram Saenger and Udo
Heinemann
Crystal structure of Homo sapiens protein hp14.5.
Proteins: Structure, Function and Bioinformatics 2004, 54: 797-800
2003
Caternia Holz, Bianca Prinz, Natalaia Bolotina, Volker Sievert, Konrad Büssow, Bernd Simon, Ulf Stahl and Christine Lang
Establishing the yeast Saccharomyces cerevisiae as a system for expression of human proteins on a proteome-scale.
Journal of Structural and Functional Genomics 2003, 4(2): 97-108.
Christoph Scheich, Volker Sievert and Konrad Büssow
An automated method for high-throughput protein purification applied
to a comparison of His-tag and GST-tag affinity chromatography
BMC Biotechology 2003, 3:12
Udo Heinemann, Konrad Büssow, Uwe Mueller, and Patrick Umbach
Facilities and methods for the high-throughput crystal structure analysis of human proteins
Accounts of Chemical Research 2003; 36(3): 157-163
2002
Konrad Büssow, Steve Hoffmann and Volker Sievert
ORFer - retrieval of protein sequences and open reading frames from GenBank and storage
into relational databases or text files
BMC Bioinformatics 2002; 3: 40
Gerald Walter, Konrad Büssow, Angelika Lueking and Jörn Glökler
High-throughput protein arrays: prospects for molecular diagnostics.
Trends in Molecular Medicine 2002; 8(6): 250-253
Joachim Klose, Christina Nock, Marion Herrmann, Kai Stühler, Katrin Marcus, Martin Blüggel,
Eberhard Krause, Leonard C. Schalkwyk, Sohaila Rastan, Steve D. M. Brown, Konrad Büssow,
Heinz Himmelbauer and Hans Lehrach
Genetic analysis of the mouse brain proteome.
Nature Genetics 2002; 30(4): 385-393
Holger Eickhoff, Zoltán Konthur, Angelika Lueking, Hans Lehrach, Gerald Walter, Eddie Nordhoff, Layos Nyarsik, Konrad Büssow
Protein array technology: the tool to bridge genomics and proteomics.
Advances in Biochemical Engineering/Biotechnology 2002; 77:103-12
2001
Konrad Büssow, Zoltán Konthur, Anglika Lueking, Hans Lehrach and Gerald Walter
Protein Array Technology: Potential Use in Medical Diagnostics.
American Journal of PharmacoGenomics 2001; 1(1): 37-43
2000
Konrad Büssow, Eckhard Nordhoff, Christine Lübbert, Hans Lehrach and Gerald Walter
A human cDNA library for high-throughput protein expression screening.
Genomics 2000; 65:1-8
Lucy J. Holt, Konrad Büssow, Gerald Walter and Ian M. Tomlinson
By-passing selection: direct screening for antibody-antigen interactions using protein arrays,
Nucleic Acids Research 2000; 28(15): E72
Gerald Walter, Konrad Büssow, Dolores Cahill, Angelika Lueking and Hans Lehrach
Protein arrays for gene expression and molecular interaction screening.
Current Opinion in Microbiology 2000; 3(3):298-302
Volker Egelhofer, Konrad Büssow, Christine Luebbert, Hans Lehrach and Eddi Nordhoff
Improvements in protein identification by MALDI-TOF-MS peptide mapping
Analytical Chemistry 2000; 72(13):2741-2750
1999
Angelika Lueking, Martin Horn, Holger Eickhoff, Konrad Büssow, Hans Lehrach and Gerald Walter
Protein microarrays for gene expression and antibody screening.
Analytical Biochemistry 1999; 270(1):103-11
Matthew D. Clark, Georgia D. Panopoulou, Dolores J. Cahill, Konrad Büssow and Hans Lehrach
Construction and analysis of arrayed cDNA libraries.
Methods in Enzymology 1999; 303:205-33
1998
Konrad Büssow, Dolores Cahill, Wilfried Nietfeld, David Bancroft, Eberhard Scherzinger, Hans Lehrach and Gerald Walter
A method for global protein expression and antibody screening on
high-density filters of an arrayed cDNA library.
Nucleic Acids Research 1998; 26(21):5007-8