Laboratory

Max-Planck-Institut für Molekulare Genetik
Proteinstrukturfabrik
Max Delbrück Centre for Molecular Medicine
Campus Berlin Buch, TRH
Robert-Rössle-Str. 10
13125 Berlin

Tel. +49 (0)30 9406-2983

Helmholtz Centre for Infection Research
Division of Structural Biology
Inhoffenstr. 7
38124 Braunschweig

Tel. +49 (0)531 6181 7064

E-Mail: buessow@molgen.mpg.de

This page: http://www.molgen.mpg.de/~buessow
Project Home Page: http://www.proteinstrukturfabrik.de/tp03page

Home Address

Schalloppstraße 21
12167 Berlin

Projects

Protein Structure Factory

At the Protein Structure Factory (PSF) - BMBF Leitprojekt Proteinstrukturfabrik -
I am responsible for the construction and characterisation of E. coli expression clones.

NGFN - German National Genome Research Network

Within the NGFN Protein Platform, my group is working on the structural analysis of human protein complexes.

Sonderforschungsbereich 633

I am taking part in the SFB 633 of the Deutsche Forschungsgemeinschaft (German Research Foundation). In this project we study E. coli proteins which are potentially involved in reactive athritis and related disorders (sub-project A4).

Nutrigenomics

PhD theses

Dissertation (PhD Thesis) of Thomas Faupel - Identification of protein-protein-interactions in vitro based on high-density protein arrays

Dissertation (PhD Thesis) of Christoph Scheich - High-throughput evaluation of protein folding conditions and expression constructs for structural genomics

Dissertation (PhD thesis) of Konrad Büssow - Arrayed cDNA libraries for antibody screening and systematic analysis of expression products

Links

Sequences and protein expression of human hEx1 cDNA library clones

Information on the JK..-markers in the EUCIB database

Links for Molecular Biology

Protocols

Publications

Download PDF reprints here.

Publications of the PSF are found here.

Jörg O. Schulze, Claudia Quedenau, Yvette Roske, Thomas Adam, Herwig Schüler, Joachim Behlke, Andrew P. Turnbull, Volker Sievert, Christoph Scheich, Uwe Mueller, Udo Heinemann and Konrad Büssow
Structural and functional characterization of human Iba proteins
FEBS Journal 2008, 275:4627-4640

Volker Sievert, Asgar Ergin, Konrad Büssow
High throughput cloning with restriction enzymes
Methods in Molecular Biology 2008, 426:163-173.

Babu A. Manjasetty, Andrew P. Turnbull, Santosh Panjikar, Konrad Büssow and Mark R. Chance
Automated technologies and novel techniques to accelerate protein crystallography for structural genomics
Proteomics 2008, 8(4): 612-625.

Sabine Cepok, Bernhard Hemmer, Konrad Büssow
Identification of Target Antigens in CNS Inflammation by Protein Array Technique
in Bioarrays - From Basics to Diagnostics. Krishnarao Appasani (Ed.), 2007.

Asgar Ergin, Konrad Büssow, Joachim Sieper, Andreas Thiel, Rainer Duchmann and Thomas Adam
Homologous high-throughput expression and purification of highly conserved E coli proteins
Microbial Cell Factories 2007, 6:18.

Christoph Scheich, Daniel Kümmel, Dimitri Soumailakakis, Udo Heinemann and Konrad Büssow
Vectors for co-expression of an unrestricted number of proteins
Nucleic Acids Research 2007, doi: 10.1093/nar/gkm067

N. S. Berrow, K. Büssow, B. Coutard, J. Diprose, M. Ekberg, G. E. Folkers, N. Levy, V. Lieu, R. J. Owens, Y. Peleg, C. Pinaglia, S. Quevillon-Cheruel, L. Salim, C. Scheich, R. Vincentelli and D. Busso
Recombinant protein expression and solubility screening in Escherichia coli: a comparative study
Acta Crystallographica Section D 2006, 62 (10): 1218-1226

Babu A. Manjasetty, Konrad Büssow, Martin Fieber-Erdmann, Yvette Roske, Johan Gobom, Christoph Scheich, Frank Götz, Frank H. Niesen and Udo Heinemann
Crystal structure of Homo sapiens PTD012 reveals a zinc-containing hydrolase fold
Protein Science 2006, 15:914-920

Claus Hultschig, Jürgen Kreutzberger, Harald Seitz, Zoltán Konthur, Konrad Büssow and Hans Lehrach
Recent advances of protein microarrays
Current Opinion in Chemical Biology 2006, 10(1):4-10.

Babu A Manjasetty, Frank H Niesen, Christoph Scheich, Yvette Roske, Frank Götz, Joachim Behlke, Volker Sievert, Udo Heinemann and Konrad Büssow
X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1)
BMC Structural Biology 2005, 5:21.

Konrad Büssow, Christoph Scheich, Volker Sievert, Ullrich Harttig, Jörg Schultz, Bernd Simon, Peer Bork, Hans Lehrach, Udo Heinemann
Structural Genomics of human proteins - target selection and generation of a public catalog of expression clones
Microbial Cell Factories 2005, 4:21.

Daniel Kümmel, Jürgen J. Müller, Yvette Roske, Rolf Misselwitz, Konrad Büssow, Udo Heinemann
The structure of the TRAPP subunit TPC6 suggests a model for a TRAPP subcomplex
EMBO reports 2005, 6(8):787–793

Sabine Cepok, Dun Zhou, Rajneesh Srivastava, Stefan Nessler, Susanne Stei, Konrad Büssow, Norbert Sommer, and Bernhard Hemmer
Identification of Epstein-Barr virus proteins as putative targets of the immune response in multiple sclerosis
Journal of Clinical Investigations 2005, 115(5):1352-60.

Hendrik Weiner, Jörg Glökler, Claus Hultschig, Konrad Büssow and Gerald Walter
Protein, antibody and small molecule microarrays, in MICROARRAY TECHNOLOGY AND ITS APPLICATIONS. ed Muller UR, Nicolau DV, Springer-Verlag Berlin, PG. 279-295. 2005.

Michael Soukenik, Anne Diehl, Martina Leidert, Volker Sievert, Konrad Büssow, Dietmar Leitner, Dirk Labudde, Linda J. Ball, Annette Lechner, Dorit K. Nägler, Hartmut Oschkinat
The SEP domain of p47 acts as a reversible competitive inhibitor of cathepsin L.
FEBS Letters 2004, 576(3):358-362.

Babu A Manjasetty, Frank H Niesen, Heinrich Delbrück, Frank Götz, Volker Sievert, Konrad Büssow, Joachim Behlke and Udo Heinemann
X-ray structure of Fumarylacetoacetate Hydrolase(FAH) family member Homo sapiens FLJ36880.
Biological Chemistry 2004, 385(10):935-942.

H. Goehler, M. Lalowski, U. Stelzl, S. Waelter, M. Stroedicke, U. Worm, A. Droege, K. S. Lindenberg, M. Knoblich, C. Haenig, M. Herbst, J. Suopanki, E. Scherzinger, C. Abraham, B. Bauer, R. Hasenbank, A. Fritzsche, A. H. Ludewig, K. Buessow, S. H. Coleman, C. A. Gutekunst, B. G. Landwehrmeyer, H. Lehrach and E. E. Wanker
A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease
Molecular Cell 2004, 15(6):853-865.

Konrad Büssow, Claudia Quedenau, Volker Sievert, Janett Tischer, Christoph Scheich, Harald Seitz, Brigitte Hieke, Frank H. Niesen, Frank Götz, Ulrich Harttig and Hans Lehrach
A catalogue of human cDNA expression clones and its application to structural genomics.
Genome Biology 2004, 5:R71.

Hendrik Weiner, Thomas Faupel and Konrad Büssow
Protein arrays for cDNA expression libraries.
Methods in Molecular Biology 2004, 278:1-14.

Christoph Scheich, Dietmar Leitner, Volker Sievert, Martina Leidert, Brigitte Schlegel, Bernd Simon, Ivica Letunic, Konrad Büssow and Anne Diehl
Fast identification of folded human protein domains expressed in E. coli suitable for structural analysis
BMC Structural Biology 2004, 4:4

Christoph Scheich, Frank Niesen, Robert Seckler and Konrad Büssow
An automated in vitro protein folding screen applied to a human dynactin subunit
Protein Science 2004, 13: 370-380

Babu A. Manjasetty, Claudia Quedenau, Volker Sievert, Konrad Büssow, Frank Niesen, Heinrich Delbrück, Udo Heinemann
X-ray structure of human gankyrin, the product of a gene linked to hepatocellular carcinoma
Proteins: Structure, Function, and Bioinformatics 2004, 55(1):214-217

Christoph Brockmann, Dietmar Leitner, Dirk Labudde, Anne Diehl, Volker Sievert, Konrad Büssow, Ronald Kühne and Hartmut Oschkinat
The solution structure of the SODD BAG domain reveals additional electrostatic interactions in the HSP70 complexes of SODD subfamily BAG domains
FEBS Letters 2004 558(1-3):101-106

Katrin de Graaf, Paul Hekerman, Oliver Spelten, Andreas Herrmann, Len C. Packman, Konrad Büssow, Gerhard Müller-Newen, and Walter Becker
Characterization of cyclin L2, a novel cyclin with an arginine/serine-rich (RS) domain: Phosphorylation by DYRK1A and colocalization with splicing factors.
Journal of Biological Chemistry 2004, 279:4612-4624

Uwe Mueller, Konrad Büssow, Anne Diehl, Franz J. Bartl, Frank H. Niesen, Lajos Nyarsik and Udo Heinemann
Rapid Purification and crystal structure analysis of a small protein carrying two terminal affinity tags.
Journal of Structural and Functional Genomics 2004, 4: 217-225

Babu A Manjasetty, Heinrich Delbrück, Dinh Trung Pham, Uwe Mueller, Martin Fieber-Erdmann, Christoph Scheich, Volker Sievert, Konrad Büssow, Frank Niesen, Wilhelm Weihofen, Bernhard Loll, Wolfram Saenger and Udo Heinemann
Crystal structure of Homo sapiens protein hp14.5.
Proteins: Structure, Function and Bioinformatics 2004, 54: 797-800

Caternia Holz, Bianca Prinz, Natalaia Bolotina, Volker Sievert, Konrad Büssow, Bernd Simon, Ulf Stahl and Christine Lang
Establishing the yeast Saccharomyces cerevisiae as a system for expression of human proteins on a proteome-scale.
Journal of Structural and Functional Genomics 2003, 4(2): 97-108.

Christoph Scheich, Volker Sievert and Konrad Büssow
An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography
BMC Biotechology 2003, 3:12

Udo Heinemann, Konrad Büssow, Uwe Mueller, and Patrick Umbach
Facilities and methods for the high-throughput crystal structure analysis of human proteins
Accounts of Chemical Research 2003; 36(3): 157-163

Konrad Büssow, Steve Hoffmann and Volker Sievert
ORFer - retrieval of protein sequences and open reading frames from GenBank and storage into relational databases or text files
BMC Bioinformatics 2002; 3: 40

Gerald Walter, Konrad Büssow, Angelika Lueking and Jörn Glökler
High-throughput protein arrays: prospects for molecular diagnostics.
Trends in Molecular Medicine 2002; 8(6): 250-253

Joachim Klose, Christina Nock, Marion Herrmann, Kai Stühler, Katrin Marcus, Martin Blüggel, Eberhard Krause, Leonard C. Schalkwyk, Sohaila Rastan, Steve D. M. Brown, Konrad Büssow, Heinz Himmelbauer and Hans Lehrach
Genetic analysis of the mouse brain proteome.
Nature Genetics 2002; 30(4): 385-393

Holger Eickhoff, Zoltán Konthur, Angelika Lueking, Hans Lehrach, Gerald Walter, Eddie Nordhoff, Layos Nyarsik, Konrad Büssow
Protein array technology: the tool to bridge genomics and proteomics.
Advances in Biochemical Engineering/Biotechnology 2002; 77:103-12

Konrad Büssow
Structural Genomics of Human Proteins at the Berlin Protein Structure Factory - Cloning and Protein Expression
Business Briefing - Future Drug Discovery 2002: 61-63

Konrad Büssow
Protein Expression and Antibody Screening.
Business Briefing - PharmaTech 2001: 152-154

Konrad Büssow
Protein Chip Technology - Biochips for the Future.
euro-biotech 2001: 16-18

Konrad Büssow
Proteins in gels, computers, crystals and camels [meeting report].
Trends in Biotechnology 2001; 19(9):328-329

Konrad Büssow, Zoltán Konthur, Anglika Lueking, Hans Lehrach and Gerald Walter
Protein Array Technology: Potential Use in Medical Diagnostics.
American Journal of PharmacoGenomics 2001; 1(1): 37-43

Konrad Büssow, Eckhard Nordhoff, Christine Lübbert, Hans Lehrach and Gerald Walter
A human cDNA library for high-throughput protein expression screening.
Genomics 2000; 65:1-8

Lucy J. Holt, Konrad Büssow, Gerald Walter and Ian M. Tomlinson
By-passing selection: direct screening for antibody-antigen interactions using protein arrays,
Nucleic Acids Research 2000; 28(15): E72

Gerald Walter, Konrad Büssow, Dolores Cahill, Angelika Lueking and Hans Lehrach
Protein arrays for gene expression and molecular interaction screening.
Current Opinion in Microbiology 2000; 3(3):298-302

Volker Egelhofer, Konrad Büssow, Christine Luebbert, Hans Lehrach and Eddi Nordhoff
Improvements in protein identification by MALDI-TOF-MS peptide mapping
Analytical Chemistry 2000; 72(13):2741-2750

Angelika Lueking, Martin Horn, Holger Eickhoff, Konrad Büssow, Hans Lehrach and Gerald Walter
Protein microarrays for gene expression and antibody screening.
Analytical Biochemistry 1999; 270(1):103-11

Matthew D. Clark, Georgia D. Panopoulou, Dolores J. Cahill, Konrad Büssow and Hans Lehrach
Construction and analysis of arrayed cDNA libraries.
Methods in Enzymology 1999; 303:205-33

Konrad Büssow, Dolores Cahill, Wilfried Nietfeld, David Bancroft, Eberhard Scherzinger, Hans Lehrach and Gerald Walter
A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library.
Nucleic Acids Research 1998; 26(21):5007-8